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上海希言科学仪器有限公司
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文献和实验: a) to the 40 ul of digested cDNA add: 1 ul Taq I adaptor (45uM) 1 ul Nco I adaptor (5uM) 2.5 ul 10 mM ATP 0.5 ul NEB T4 DNA ligase buffer 0.2 ul T4 DNA ligase 45 ul total b) 37C for 3hrs or ON @ 30C
Preparation of Carboxypeptidase Y and Its Properties
to convert zymogen into active enzyme. Secondly, chromatography on DEAE-cellulose is used to remove other proteins. Finally, chromatography on DETA-Sephadex A-50 and Sephadex G-150 are applied to purify further the enzyme, and the enzyme is identified
0.1% SDS 50mM Tris pH 7.4-7.5 150mM NaCl 10% glycerol 1mM EDTA Modified RIPA Buffer - Good general-purpose buffer 1% NP40 0.5% NaDOC 50mM Tris pH 7.5 150mM NaCl 5mM EDTA NP40 Buffer
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