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文献和实验Lipoprotein Isolation - First week
ml plastic syringe (held by a clamp) attached to a 18 gauge needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay
Lipoprotein Isolation - First week
more (2-3 ml) PBS to the undissolved KBr and stir until dissolved. Pool these solutions and make up to 20 ml with PBS and stir further in the measuring cylinder. 9. Transfer solution into a 30 ml plastic syringe (held by a clamp) attached to a 18 gauge
PREPARATION OF SEQUENCING GELS
MATERIALS: 2-glass plates 1 sharks -tooth comb and spacers Whatman 3 mm paper 30 or 40% acrylamide-bis (19:1) 10X TBE urea 10% ammonium persulfate TEMED 60 cc. syringe Rain-ex Preparation of glass plates: Wash and rinse
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