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文献和实验Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids
Exosomes are 40–100 nm sized vesicles released from cells when multivesicular bodies fuse with the plasma membrane. These vesicles take part in cell-to-cell communication by binding and signalling through membrane receptors on cells
Method for Single-Cell Electroporation
transferred into the cell through these pores. Following pulse termination, pores reseal over 10s to 100s of ms. SCE is a powerful transfection method since it readily allows the delivery of multiple genes each carried by independent plasmids
. Resuspend cells in appropriate medium or buffer. Quantitate cell yield and viability. Seed cells for culture, if required. Perfusion procedures require special equipment and techniques
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