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文献和实验Immunofluorescent Staining of Drosophila Larval Brain Tissue
(Dpn; types I and II; green) and Asense (Ase; type I only; blue), and the cortical marker Discs large (Dlg; red). (A ) Wild-type brain showing Dpn+ Ase+ type I neuroblasts (white arrowheads) and Dpn+ Ase- type II neuroblasts (black arrowheads). (B
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, the DCC has been used to study neurite outgrowth(12). We have previously used the DCC to elucidate the role of Rho family proteinsin the chemotactic response of BAC1.2F5 macrophages to CSF-1 (13). Wedescribe the use of this chamber to study the response
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of PCR are performed followed by PCR products cloning and sequencing. (b) SplinkBlunt-PCR protocol. Genomic DNA (horizontal lines) is digested with restriction enzymes (X, Y, and Z in this case)in a similar way described in (a). After the digested double
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