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文献和实验Lipid analysis Week 3: GAS LIQUID CHROMATOGRAPHY
a temperature program: For the first part of the run, the column temperature is low; after the short fatty acids have passed the column, the temperature is gradually increased until all components have left. The interaction of the mobile phase depends
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
: For the first part of the run, the column temperature is low; after the short fatty acids have passed the column, the temperature is gradually increased until all components have left. The interaction of the mobile phase depends on its chemical nature. Non-polar phases
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
. However, short fatty acids will then not separate. If one is interested in achieving good separation of both, short and long fatty acid methyl esters, is is advantageous to use a temperature program: For the first part of the run, the column temperature is low
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