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文献和实验, the tubing used to transfer your high-grade water should always be the most inert available, typically TeflonTM or similar materials. Never use highly plasticized flexible plastic tubing. Absolutely avoid metals such as copper or stainless steel
. For the same reason, the tubing used to transfer your high-grade water should always be the most inert available, typically TeflonTM or similar materials. Never use highly plasticized flexible plastic tubing. Absolutely avoid metals such as copper or stainless steel
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
. Approximately 2 mm from the end, bend the wire at a 45° angle (see Fig. 1 , top electrode). Using clear nail polish, insulate the wire near the junction where the cathode will be mounted on the micromanipulator. Couple the cathode to a stainless steel rod
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