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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
, using 400x in phase contrast mode. Can you see one or more flagella? Add a drop or two of Lugol's iodine to what remains in the culture tube (ratio should be 4:1 culture to iodine). Mix and prepare a slide of one drop of culture, with cover slip
Sphingomyelin Mass Measurement
Protocol: Bligh & Dyer extraction 1) Pellet approximately 1 X 107 cells. 2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard. 3) Add 0.8 ml H2O, vortex hard and allow solution to sit at room temperature for atleast 30
Sphingomyelin Mass Measurement
) for 5 minutes. 5) Transfer solvents to a new tube without transferring any of the pelleted debris. 6) Add 1 ml of CHCl3 and 1 ml of H2O to each sample. --> Upon addition of CHCl3, a biphasic solution should result. 7) Vortex hard and spin samples
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