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文献和实验screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels. 6) Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way
2 1 μl dNTP mix (10 mM each nt) 2.5 μL Forward Primer (10 μM stock) 2.5 μL Reverse Primer (10 μM stock
Optimal arbitrary primer length for Differential Display(图)
The concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to systematically amplify and visualize most of the mRNA in a cell. The optimal length of arbitrary primers
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