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上海希言科学仪器有限公司
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文献和实验by combining a small amount of a Wnt samplewith 4× loading dye and water to give a final concentrationof the loading dye of 1× in a total volume of 20 to 40μL. Use the following amounts of Wnt per 20 μL sample: 1)Wnt3A CM: 1 μL; 2) Blue Sepharose fractions: 1 μ
below to preparethe reaction mixture for each pair of oligos to phosphorylateand anneal them into ligation-ready duplex DNA fragments. Place the mixture in a thermocycler using the followin parameters to phosphorylate and anneal the oligos. Add 2 μl
-regenerating system Prepare cytosol mix (volumes correspond to one reaction of 50 μl, scale up accordingly): 100 μg cytosol (usually 10�17 μl, depending on the concentration), 0.9 μl DHM buffer, 2.25 μl KAc buffer, 1.67 μl ATP solution
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