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上海希言科学仪器有限公司
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文献和实验2) approximately 10 mg FFPE sample add 95 ul FP1 Buffer and 5 μl FP2 to the collection tube. Close the tube and vortex briefly. Make sure the sample is covered by the Extraction Solution. 3) Incubate at 60°C for 30-60 minutes. 4) Incubate at 90°C
Maintenance of Chicken Embryonic Stem Cells In Vitro
as a fusion protein linked to glutathione S-transferase (GST) and purified to greater than 90% purity in two chro-matography stages, the first an affinity step using the GST tail, which was cleaved before further purification by gel chromatography. Chicken ES
, should be used. 2. Add 80 ul Buffer DS and vortex to mix. 3. Incubate at 70°C for 10 min. Briefly vortex the tube once during the incubation. For some difficult lysis bacterial, Increase the temperature to 90°C. 4. Centrifuge at 13,000 x g
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