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文献和实验Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
address. (C ) Standard Web browser on the host computer (here, Mozilla Firefox on Mac OS X). ProHits Lite VM may be accessed by typing the IP address from panel B into the host computer browser. Other computers on the network can access the VM
Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
Yeast Chromatin Immunoprecipitation (ChIP)
buffer+protease inhibitors to make 50% slurry (prepare 25% more beads than you calculate to be necessary) 7.Add 60μl of 50% bead slurry to each IP sample,nutate in cold room 1hr 8.Spin beads down in cold room microcentrifuge for 10-15 sec,remove
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