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文献和实验) and 2ml 7 x annealing buffer. Heat the mixture to 65℃ for 2 minutes and allow to cool slowly, over a period of about 30 minutes, to roo m temperature. 4) While the annealing reaction is taking place, take 4 sterile microcentrifuge tubes per sample
stock) and 2ml 7 x annealing buffer.Heat the mixture to 65°C for 2 minutes and allow to cool slowly,over a period of about 30 minutes,to roo m temperature. 4)While the annealing reaction is taking place, take 4 sterile microcentrifuge tubes per sample
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
plate, wash excess w/ EtOH. Taping is unnecessary, use 3 sided spacers and plug w/ 2ml gel mix + 2ul TEMED in pasteur pipette. 2) Gels: Acryl stocks are filter sterilized. First warm urea in H2O to dissolve. Cast gels last 5-7d RT, are made 0.5 x
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