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文献和实验3X Flag Peptide) to each pelleted bead/complex, incubate 30-60 minutes at 4°C with gentle shaking;Centrifuge the resin for 30 seconds at 10,000 RPM, transfer the supernatants (which should now contain the ChIP/Flag eluted complexes) to fresh tubes
Native Chromatin Preparation and Illumina/Solexa Library Construction
Microcentrifuge tubes Micropipettor and tips Rotator Sonicator Spectrometer (Qubit Systems) Test tubes, conical, 50- and 250-mL Thermal cycler Tubes (microcentrifuge) Vortex mixer Water baths or heating
Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
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