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上海希言科学仪器有限公司
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文献和实验Single tube confirmation PCR protocol
l Taq Polymerase (2.5 units) 638 µl 22 X 29 µl water 5 µl 10 µM upstream primer: A,C,or kanC (1 µM) 5 µl 10 µM downstream primer:B,kanB,or D(1 µM) 5 µl Zymolyase treated cells
the supplemented Cell Line Solution V to room temperature. Pre-warm one 500 μl aliquot of growth medium per sample (in a sterile microfuge tube) at 37°C. Prepare 6-well plates with 2 ml culture medium and pre-incubate the plates in a humidified 37°C
chromatin while keeping samples on ice (1 interval for 20 seconds at power setting of "3" on the Branson 50 cell machine). Immediately centrifuge at 14,000 RPM for 10 minutes at 4°C;Carefully remove the supernatant and transfer to a new tube. Preclear
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