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文献和实验96-well RNA In Situ Hybridization Protocol
phase along with embryos and vitelline membranes.remaining at the interphase.9. Rinse settled embryos 3X with methanol (Embryos can be stored at -20 degrees C at this point).10. Rehydrate in 3:1 (MeOH: 4% paraformaldehyde/PBS) for 2 min., then 1:3 (MeOH
out 400 µl portions of the column eluate to 1.5 ml Eppendorf tubes. 23. Add 1/10 volume of 3M sodium acetate (pH 5.2). 24. Add 1 ml of ethanol to each tube. 25. Let stand for 15 minutes. 26. Centrifuge at 12000 x g at 4C for 15
Lacrimal Experimental Protocol (4/4/94)
6 for 6 min on Eppendorf centrifuge. Retain supernatant and store at -70°C. Make up trypsin/EDTA/dispase by adding 0.9 ml of 10 x trypsin/EDTA to 3.6 ml of dispase; put at 37°C 9. After 100 min, remove and retain stimulation media. Add 100 µl
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