phospho-p70 S6K (Thr389) ELISA kit 85-86052-11
Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse p70 S6K in cell lysates. Detection of rat p70 S6K is expected.The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. p70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.
Reactivity
Human, Mouse
Components
User Manual InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed) Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL Enhancer Solution (Part No. IOES1) - 0.5 mL Wash Buffer (10X) (Part No. IOWB1) - 8 mL phospho-p70 S6K (Thr389) Capture Antibody Reagent (Part No. IOPS6KCAR1) - 3 mL phospho-p70 S6K (Thr389) Detection Antibody Reagent (Part No. IOPS6KDAR1) - 3 mL Positive Control Cell Lysate (lyophilized) (Part No. IOS6KPC1) - 1 ea Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL Stop Solution (Part No. IOSS1) - 10 mL Plate seal - 1 ea