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15.63-1000pg/mL
elisa
科学研究
人
serum, plasma and other biological fluids.
1周
研卉生物
96T
Organism species | Homo sapiens (Human) |
Product No. | SEB695Hu |
Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Format | 96-well strip plate |
Assay length | 4.5 hours |
Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
Sensitivity | The minimum detectable dose of this kit is typically less than 0.49pg/mL. |
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 87-95 | 90 |
EDTA plasma(n=5) | 96-104 | 101 |
heparin plasma(n=5) | 84-95 | 91 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-105% | 84-103% | 79-97% | 84-93% |
EDTA plasma(n=5) | 80-101% | 78-89% | 91-101% | 85-104% |
heparin plasma(n=5) | 96-105% | 78-96% | 96-105% | 80-91% |
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
参考文献 | Evaluation of Th17-related cytokines and receptors in multiple sclerosis patients under interferon beta-1 therapy |
引用杂志 | Journal of Neuroimmunology 阅读[PubMed: 23177721] |
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