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- BioVision
- 美国
- 1993-1
- 2026年05月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见产品包装
- 保质期：
一年
- 英文名：
N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide; Tzv
- 库存：
大量
- 供应商：
QIYBO
- 规格：
1 ml
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文献和实验BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate
Preparing a Selenomethionyl Protein
50x stock Use all amino acids EXCEPT Gly, Ala, Pro, Asn, Cys, and Met at a concentration of 2mg/ml To help in dissolving the amino acids, autoclave for 10 minutes. 20% glucose (sterile filtered or autoclaved) 1M MgSO4 (sterile
Preparing a Selenomethionyl Protein
for 10 minutes. 20% glucose (sterile filtered or autoclaved) 1M MgSO4 (sterile filtered) 2M CaCl2 (sterile filtered) 0.5% (w/v) Thiamine Solution (sterile filtered) Procedure Day 1 Prepare a 2mL day culture
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