- ￥900 - 1800
- 华拓生物
- 中国
- HT1610961
- 2025年07月10日
- FC
- Mouse
- Hu
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD45RA
- 亚型：
Mouse IgG2a
- 形态：
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件：
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性：
Monoclonal Antibody
- 标记物：
PerCP-CY5.5
- 适应物种：
Hu
- 宿主：
Mouse
- 应用范围：
FC
- 浓度：
Concentration：0.5 mg/ml
- 靶点：
Clone number：HI100
- 抗体英文名：
CD45RA Antibody-PerCP-CY5.5；流式抗体
- 抗体名：
CD45RA Antibody-PerCP-CY5.5；流式抗体
- 规格：
25/100 tests
克隆类型 ：Mouse Monoclonal Antibody；Clone number：HI100
货 号 ：HT1610961
规 格 ：25/100 tests;
亚 型 ：
Mouse IgG2a
浓 度 ：
Concentration：0.5 mg/ml
缓 冲 液 ：
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 ：
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naive T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
适用物种 ：
Hu
应用范围 ：
FC
应用说明 ：
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 ：
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 ：
PerCP-CY5.5（其他荧光标记请咨询我们）
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文献和实验Basic Method for Direct Immunofluorescence Labeling
Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you're planning to label cells with 2 or more antibodies simultaneously, you need a negative control for each fluorochrome
Basic Method for Direct Immunofluorescence Labeling
in a fluorochrome-conjugated form; Fluorochrome conjugation interferes with the specificity of the antibody; The number of receptors per cell is low (indirect methods generally can render brighter labeling); You need very accurate comparisons
7+CD45RA-）， 效应记忆 T 细胞（CCR7-CD45RA-）。对 CD4+ 细胞 CD4/CD25 作图可分析调 T 表达，对 CD4+CD25+ 的细 胞进一步设门至 CD127/CD45RA，分析天然调 T 细胞（CD127+CD45RA+）以及记忆调 T 细胞（CD127+CD45RA-）， 效应调 T 细胞（CD127 hi+CD45RA-）。T8 细胞的成熟过程及活化可以用 CD27/CD28，CD57/CD279 散点图分析。 纯真 T 细胞（CD3+CCR7+CD45RA
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