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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD11c
- 亚型:
Mouse IgG1
- 形态:
Aqueous buffer, 0.09% NaN3, may contain carrier protein/stabilize.
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:3.9
- 抗体英文名:
CD11c Antibody-PE;流式抗体
- 抗体名:
CD11c Antibody-PE;流式抗体
- 规格:
25/100 tests
克隆类型 :Mouse Monoclonal Antibody;Clone number:3.9
货 号 :HT1610787
规 格 :25/100 tests;
亚 型 :
Mouse IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
Aqueous buffer, 0.09% NaN3, may contain carrier protein/stabilize.
产品描述 :
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx, and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used 20 µL to 5 µL /10^6 cells or 100 µL of whole blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE(其他荧光标记请咨询我们)
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文献和实验A Survey of Copy‐Number Variation Detection Tools Based on High‐Throughput Sequencing Data
mapping and assembly of structural variant breakpoints in the mouse genome. Genome Res. 20:623‐635. Ramachandran, A., Micsinai, M. and Pe'er, I. 2011. CONDEX: Copy number
Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
,普遍认为(也可能是我自己认为),第6天或第8天收不成熟DC会比较多,看实验需要,建议想要不成熟DC的朋友请在这两天收网)。 我一般做到第5步就上流式,也就是6到8天的BMDC, CD11c表达波动在50-80%,偶尔做CD86,成熟标志吧?15%左右。具体流式结果和光镜形态照片过几天(或几周)发上来大家一起探讨。MHC2我没有做(技术员说实验室流式机器做APC的有关部件有故障,我的MHC2抗体正好带APC)。还发现用PE标记和FITC标记的CD11c流式抗体检测CD11c表达发现阳性
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