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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD8
- 亚型:
Mouse IgG1
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
APC
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:HIT10a
- 抗体英文名:
CD8 Antibody-APC;流式抗体
- 抗体名:
CD8 Antibody-APC;流式抗体
- 规格:
25/100 tests
克隆类型 :Mouse Monoclonal Antibody;Clone number:HIT10a
货 号 :HT1610715
规 格 :25/100 tests;
亚 型 :
Mouse IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
APC(其他荧光标记请咨询我们)
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文献和实验Production of CD4+ and CD8+ T Cell Hybridomas
T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated
#01 什么是同型对照? 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照? 流式抗体与靶标的结合,是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞(除T细胞外)表面大多表达 CD16、CD32、CD64 等 Fc 受体,这些受体可与各种抗体的 Fc 端非特异性结合,出现非特异
样本与特定实验条件下的标本之间的差别。例如,在研究某种药物对某细胞株的影响时,需要设置未经药物处理的相同细胞株作为实验对照。 示例:完整的 CD3-APC、CD4-FITC、CD8-PE 的 3 色实验样本管设置
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