生物活性
Sal003 is a potent, specific and cell-permeable inhibitor of the eukaryotic translation initiation factor 2α (eIF2α) phosphatase. Sal003 is a derivative of salubrinal[1].
IC50 & Target
eIF2α phosphatase[1]
体外研究(In Vitro)
Sal003 (20 μM; 1-12 hours) sharply increases eIF2α phosphorylation in mouse embryonic fibroblasts (MEFs)[2].
Eukaryotic translation initiation factor 2α (eIF2α) phosphorylation by Sal003 (10 μM; 1 hour) enhances subtilase cytotoxin (SubAB)-induced apoptotic signaling[1].
Sal003 promotes eIF2α phosphorylation leads to impairment of synaptic plasticity and memory[1].
Apoptosis Analysis[2]
Cell Line: HeLa cells
Concentration: 10 μM
Incubation Time: 1 hour
Result: Phosphorylated eIF2α and thus enhanced SubAB-induced apoptotic signaling.
体内研究
(In Vivo)
Sal003 (20Μm; intrahippocampal injection; 8 minutes) impairs contextual memory in vivo[1].
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:
DMSO : ≥ 100 mg/mL (215.88 mM)
* "≥" means soluble, but saturation unknown.
配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.1588 mL 10.7942 mL 21.5885 mL
5 mM 0.4318 mL 2.1588 mL 4.3177 mL
10 mM 0.2159 mL 1.0794 mL 2.1588 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: