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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD44
- 亚型:
Rat IgG2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PE-CY7
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.2 mg/ml
- 靶点:
Clone number:IM7
- 抗体英文名:
CD44 Antibody-PE-CY7;流式抗体
- 抗体名:
CD44 Antibody-PE-CY7;流式抗体
- 规格:
25/100 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:IM7
货 号 :HT1610215
规 格 :25/100 μg ;
亚 型 :
Rat IgG2b
浓 度 :
Concentration:0.2 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
the suggested use of this reagent for immunofluorescent staining is ≤0.25 µg per 10^6 cells in 100 µl volume.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PE-CY7(其他荧光标记请咨询我们)
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
+ 1% BSA, + 5% heat inactivated normal rabbit serum (HINRS). 9) Label with antibodies to surface molecules that are resistant to fixation (30 mins 4 deg C) eg. anti-CD4 (H129.19-Quantum Red; Sigma R 3637) for FL3 anti-CD44 (Pgp-1:IM7
Fluorochrome Absorption and Emission Spectra
) and approximately half of the absorbed photons are emitted as fluorescent light. The number of FITC molecules per conjugate partner (antibody, Avidin, Streptavidin, etc.) is usually in the range of three to five molecules. R-phycoerythrin (R-PE
Fluorochrome Absorption and Emission
) and approximately half of the absorbed photons are emitted as fluorescent light. The number of FITC molecules per conjugate partner (antibody, Avidin, Streptavidin, etc.) is usually in the range of three to five molecules. R-phycoerythrin (R-PE
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