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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD8
- 亚型:
Rat IgG2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
PerCP-CY5.5
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.2 mg/ml
- 靶点:
Clone number:YTS169.4
- 抗体英文名:
CD8 Antibody-PerCP-CY5.5;流式抗体
- 抗体名:
CD8 Antibody-PerCP-CY5.5;流式抗体
- 规格:
25/100 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:YTS169.4
货 号 :HT1610093
规 格 :25/100 μg ;
亚 型 :
Rat IgG2b
浓 度 :
Concentration:0.2 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 1.0 µg /10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
PerCP-CY5.5(其他荧光标记请咨询我们)
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文献和实验的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。 不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5
的功能决定的,所以首先需要注意两点: 流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光,能做FL1、FL2、FL3三个荧光通道/三个颜色,而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。 不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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