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特点: 银 (Ag) 离子抗菌表面可抑制细菌、微生物和真菌的生长 “Set-and-forget(设置防遗忘)”移液按钮可以方便的进行容量调节 移液按钮在非锁定位置时可以自由旋转 指托可调节 120° 角度以寻求最佳的移液位置 质柔、平稳的移液操作可在较长时间的移液过程中获得更好的结果 50μL 体积及以下的超级吹出活塞确保微量液滴的排出 专利的轻触式吸头弹出可最大限度地减少所需的吸头弹力
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文献和实验) - mix 16 µg MseI-oligo.1 and 14 µg MseI-oligo.2, 3 µl 10X One-Phor-All+,Q.S. to 60 µL w/ dH2O, heat to 95° C, and allow to cool to RT slowly. This gives a final concentration of 50 pmoles/µL and makes enough adapter for 60 ligations. [Note
AFLP: not only for fingerprinting, but for positional cloning
extensions one will have 16 independent primers.] 2.2 AFLP-PCR AFLP cocktail (recipe is for one rxn) 0.5 µL labeled EcoRI-primer (@ 10 ng/µL) 0.6 µL unlabeled MseI-primer‡ (@ 50 ng/µL) 0.8 µL 5 mM dNTPs 2.0 µL 10x-Promega Taq buffer (Mg-free
AFLP: not only for fingerprinting, but for positional cloning
min. Pellet nucleic acids by centrifugation (12K x g, 5 mn). [This step will remove chlorophyll as well as any debris carried over from step 4.] 6) Wash pellet w/ 75% EtOH, dry and resuspend in 10-50 µL TE (pH 7.5). 7) Quantitate nucleic
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