手机验证
充足
cloud-clone corp(优尔)
19.5-5,000ng/mL
竞争抑制
详见说明
Mus musculus (Mouse,小鼠)
详见说明
Plasma
48T
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将D-二聚体(D2D)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的D-二聚体(D2D)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。
杂志 | 参考文献 |
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D-二聚体(D2D)检测试剂盒(化学发光免疫分析法)说明书.pdf 附件下载 (下载 1 次)
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