手机验证
充足
杭州昊鑫生物
15.6-1,000pg/mL
双抗夹心
详见说明
Rattus norvegicus (Rat,大鼠)
详见说明
serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
48T
本试剂盒用于检测转化生长因子β1(TGFb1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的转化生长因子β1(TGFb1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 96-103 | 101 |
EDTA plasma(n=5) | 83-93 | 89 |
heparin plasma(n=5) | 83-104 | 101 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的转化生长因子β1(TGFb1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中转化生长因子β1(TGFb1)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-97% | 97-104% | 95-105% | 98-105% |
EDTA plasma(n=5) | 96-103% | 83-96% | 78-93% | 79-101% |
heparin plasma(n=5) | 92-101% | 78-101% | 88-103% | 86-98% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将转化生长因子β1(TGFb1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的转化生长因子β1(TGFb1)与连接于固相载体上的抗体结合,然后加入生物素化的转化生长因子β1(TGFb1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的转化生长因子β1(TGFb1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
Cardiovascular Research | Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway [OxfordJournals: source] |
Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid [ScienceDirect: S0188440910001049] |
Biochemical and Biophysical Research Communications | Chronic angiotensin-(1–7) administration improves vascular remodeling after angioplasty through the regulation of the TGF-β/Smad signaling pathway in rabbits [ScienceDirect: S0006291X09017021] |
European Cells and Materials | Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation. [Pubmed: 22314694] |
Journal of Animal and Veterinary Advances | A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivo [Medwell: 2011642650] |
revue de medecine veterinaire | Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosis [Revmedvet: Source] |
Biomedical and Environmental Sciences | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats [Besjournal: Source] |
The Open Respiratory Medicine Journal | Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias [PubMed: PMC3551240] |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
J Physiol Pharmacol. | Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet [Pubmed: 23568967] |
international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
Journal of ethnopharmacology | Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397] |
Int J Clin Exp Pathol | Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration [Ijcep:Source] |
Am J Physiol Regul Integr Comp Physiol. | Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: Role of TGFβ and TNFα [Pubmed:25715833] |
Exp Ther Med. | PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis [Pubmed:Pmc4079423] |
International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396] |
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杂志 | 参考文献 |
Cardiovascular Research | Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway [OxfordJournals: source] |
Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid [ScienceDirect: S0188440910001049] |
Biochemical and Biophysical Research Communications | Chronic angiotensin-(1–7) administration improves vascular remodeling after angioplasty through the regulation of the TGF-β/Smad signaling pathway in rabbits [ScienceDirect: S0006291X09017021] |
European Cells and Materials | Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation. [Pubmed: 22314694] |
Journal of Animal and Veterinary Advances | A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivo [Medwell: 2011642650] |
revue de medecine veterinaire | Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosis [Revmedvet: Source] |
Biomedical and Environmental Sciences | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats [Besjournal: Source] |
The Open Respiratory Medicine Journal | Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias [PubMed: PMC3551240] |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
J Physiol Pharmacol. | Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet [Pubmed: 23568967] |
international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
Journal of ethnopharmacology | Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397] |
Int J Clin Exp Pathol | Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration [Ijcep:Source] |
Am J Physiol Regul Integr Comp Physiol. | Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: Role of TGFβ and TNFα [Pubmed:25715833] |
Exp Ther Med. | PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis [Pubmed:Pmc4079423] |
International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396] |