手机验证
充足
cloud-clone corp(优尔)
0.156-10ng/mL
双抗夹心
详见说明
Sus scrofa; Porcine (Pig,猪)
详见说明
Serum, plasma and other biological fluids
48T
本试剂盒用于检测免疫球蛋白E(IgE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的免疫球蛋白E(IgE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 90-99 | 96 |
EDTA plasma(n=5) | 91-98 | 94 |
heparin plasma(n=5) | 80-93 | 88 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的免疫球蛋白E(IgE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中免疫球蛋白E(IgE)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-105% | 78-97% | 86-94% | 92-105% |
EDTA plasma(n=5) | 88-97% | 78-89% | 86-96% | 78-97% |
heparin plasma(n=5) | 93-101% | 95-103% | 78-101% | 91-98% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将免疫球蛋白E(IgE)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的免疫球蛋白E(IgE)与连接于固相载体上的抗体结合,然后加入生物素化的免疫球蛋白E(IgE)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的免疫球蛋白E(IgE)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
Molecular Carcinogenesis | IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection [PubMed: 23280722] |
Human Immunology | IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection [PubMed: 23354321] |
Scand J Immunol | Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation [Pubmed: 24313384] |
AAPS PharmSciTech | Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806] |
Ann Allergy Asthma Immunol | Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423] |
Molecular Medicine Reports | The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. [pubmed:27484042] |
Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
International Immunopharmacology | Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791] |
Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
Water Research | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
Human & Experimental Toxicology | Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles [Pubmed:29233007] |
Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
Journal Of Cellular Biochemistry | Effects of catalpol on bronchial asthma and its relationship with cytokines [Pubmed: 30536454] |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
Health and Medicine | Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis [] |
American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
international journal of antimicrobial agents | Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278] |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
杂志 | 参考文献 |
Molecular Carcinogenesis | IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection [PubMed: 23280722] |
Human Immunology | IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection [PubMed: 23354321] |
Scand J Immunol | Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation [Pubmed: 24313384] |
AAPS PharmSciTech | Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806] |
Ann Allergy Asthma Immunol | Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423] |
Molecular Medicine Reports | The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. [pubmed:27484042] |
Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
International Immunopharmacology | Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791] |
Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
Water Research | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
Human & Experimental Toxicology | Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles [Pubmed:29233007] |
Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
Journal Of Cellular Biochemistry | Effects of catalpol on bronchial asthma and its relationship with cytokines [Pubmed: 30536454] |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
Health and Medicine | Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis [] |
American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
international journal of antimicrobial agents | Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278] |