手机验证
充足
杭州昊鑫生物科技股份有限公司
0.312-20ng/mL
双抗夹心
详见说明
Homo sapiens (Human,人)
详见说明
serum, plasma, tissue homogenates and other biological fluids
96T
本试剂盒用于检测二胺氧化酶(ABP1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的二胺氧化酶(ABP1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中二胺氧化酶(ABP1)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 99-105% | 78-88% | 78-102% | 94-102% |
EDTA plasma(n=5) | 78-103% | 91-101% | 89-97% | 96-103% |
heparin plasma(n=5) | 84-91% | 92-105% | 81-97% | 96-104% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将二胺氧化酶(ABP1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的二胺氧化酶(ABP1)与连接于固相载体上的抗体结合,然后加入生物素化的二胺氧化酶(ABP1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的二胺氧化酶(ABP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
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