血管生成素2(ANGPT2)检测试剂盒(酶联免疫吸附试验法)

血管生成素2(ANGPT2)检测试剂盒(酶联免疫吸附试验法)

优尔生一级代理：杭州昊鑫生物
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优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真核（酵母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。

• 编号SEA009Po
• 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
• 实验方法双抗夹心
• 反应时长3h
• 检测范围1.56-100ng/mL
• 灵敏度最小可检测剂量小于等于0.54ng/mL.
• 样本类型Serum, plasma and other biological fluids
• 下载英文说明书 中文说明书
• 规格48T96T 96T*5 96T*10 96T*10

产品包装图模拟

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管生成素2(ANGPT2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管生成素2(ANGPT2)与连接于固相载体上的抗体结合，然后加入生物素化的血管生成素2(ANGPT2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管生成素2(ANGPT2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

参考文献

杂志	参考文献
Microvascular Research	Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle [PubMed: 20937289]
Theriogenology	Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
Pediatr Infect Dis J	Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever [PubMed: 25831422]
Oncotarget	Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways [Pubmed:26717040]
Experimental Animals	Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. [pubmed:28367863]
Sci Rep.	Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJ	The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Cellular Physiology and Biochemistry	Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats [pubmed:28848201]
Tumor Biology	Cervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Anesthesiology	Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
British Journal of Anaesthesia	Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
Anticancer Research	Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway [Pubmed: 30711932]
Comparative Medicine	Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female … [Pubmed: 31526432]
BMC Endocrine Disorders	The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
Biology of Reproduction	Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 [Pubmed: 31806575]
Scientific Reports	Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
MEDIATORS OF INFLAMMATION	Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7 … [Pubmed: 33100902]
Scientific Reports	Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735]
Intensive Care Med Exp	The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats [33997943]
Gynecol Endocrinol	Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study [34282706]