手机验证
充足
杭州昊鑫生物
0.156-10ng/mL
双抗夹心
详见说明
Rattus norvegicus (Rat,大
详见说明
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T
本试剂盒用于检测细胞色素P450家族成员2E1(CYP2E1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的细胞色素P450家族成员2E1(CYP2E1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 86-96 | 90 |
EDTA plasma(n=5) | 83-97 | 93 |
heparin plasma(n=5) | 81-101 | 88 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的细胞色素P450家族成员2E1(CYP2E1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中细胞色素P450家族成员2E1(CYP2E1)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-104% | 98-105% | 78-104% | 90-103% |
EDTA plasma(n=5) | 83-102% | 78-101% | 92-103% | 79-97% |
heparin plasma(n=5) | 92-101% | 94-105% | 96-104% | 79-94% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将细胞色素P450家族成员2E1(CYP2E1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞色素P450家族成员2E1(CYP2E1)与连接于固相载体上的抗体结合,然后加入生物素化的细胞色素P450家族成员2E1(CYP2E1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞色素P450家族成员2E1(CYP2E1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
BMC Complementary and Alternative Medicine | Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats [PubMed: PMC3605171] |
Evidence-Based Complementary Alternative Medicine | Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats [PubMed: PMC3749608] |
BMC Complementary and Alternative Medicine | CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat [PubMed: PMC3519517] |
Asian Journal of Biochemical and Pharmaceutical Research | Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source] |
The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
Journal of Functional Foods | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo [publication:307092820] |
CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA | Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats. [59f7c85aaca272607e2d8d7a] |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
Nutrients | Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages [Pubmed: 31935882] |
Antioxidants | Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress [34573017] |
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杂志 | 参考文献 |
BMC Complementary and Alternative Medicine | Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats [PubMed: PMC3605171] |
Evidence-Based Complementary Alternative Medicine | Mechanism of Hepatoprotective Effect of Boesenbergia rotunda in Thioacetamide-Induced Liver Damage in Rats [PubMed: PMC3749608] |
BMC Complementary and Alternative Medicine | CCl4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat [PubMed: PMC3519517] |
Asian Journal of Biochemical and Pharmaceutical Research | Hepatoprotective Effect of Methanolic Stem Extract of Bush Cane (Costus Afer) on Immunologic Response Generated Reactive Oxygen Species (ROS) in Alcohol Induced Liver Cirrhosis in Rats. [Ajbpr:Source] |
The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
Journal of Functional Foods | Protective effect of Djulis (Chenopodium formosanum) and its bioactive compounds against carbon tetrachloride-induced liver injury, in vivo [publication:307092820] |
CIENC TEC VITIVINIC,CIENCIA E TECNICA VITIVINICOLA | Haloperidol loaded carboxymethyl chitosan nanoparticles reduce Oxidative stress, Mitochondria dysfunction and DNA damage in hepatic cells of male rats. [59f7c85aaca272607e2d8d7a] |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
Nutrients | Oxidative Stress Parameters in the Liver of Growing Male Rats Receiving Various Alcoholic Beverages [Pubmed: 31935882] |
Antioxidants | Ellagic Acid Prevents Binge Alcohol-Induced Leaky Gut and Liver Injury through Inhibiting Gut Dysbiosis and Oxidative Stress [34573017] |