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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Spindlin-2B (SPIN2B)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Spindlin-2B (SPIN2B)品名简称:
Recombinant Human SPIN2B protein货号:
CSB-EP859828HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
SPIN2BUniprot No.:
Q9BPZ2别名:
Spindlin-like protein 2B种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
56.2 kDa表达区域:
1-258aa氨基酸序列:
MKTPNAQEAEGQQTRAAAGRATGSANMTKKKVSQKKQRGRPSSQPRRNIVGCRISHGWKEGDEPITQWKGTVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGEHGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLIGKHVEYTKEDGSKRIGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKKS蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the regulation of cell cycle progression, this activity is related to the inhibition of apoptosis following the removal of essential growth factors.功能2:
Involved in the regulation of cell cycle progression, this activity is related to the inhibition of apoptosis following the removal of essential growth factors文献:
"Functional cloning of SPIN-2, a nuclear anti-apoptotic protein with roles in cell cycle progression." Fletcher B.S., Dragstedt C., Notterpek L., Nolan G.P. Leukemia 16:1507-1518(2002)研究领域:
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文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
:actin, c-Abl, cyclin B1, MDM2/p53, IκB, MPF, PKA, Rev和MEK,会受到Leptomycin B的影响 Leptomycin B是用于研究很多调节蛋白亚细胞定位的非常有效的工具。核―质间的运输障碍和很多疾病相关,包括自身免疫缺陷性疾病和癌症。从发表在在PUBMED上的相关文章我们可以发现,用Leptomycin B进行的研究几乎涉及了所有的常见的细胞蛋白,包括:p53, cyclins, protein kinases, and steroid
[好书下载] Protein-Protein Interactions--Methods and Applications
标题:Protein-Protein Interactions--Methods and Application内容:研究蛋白质相互作用的各种方法和应用格式:PDF大小:10Mb(最小不要小于500kb)语种:英文简介:2004年的humana press出版的,为目前有关蛋白质相互作用的方法总结下面是该书的目录Protein–Protein Interactions :Methods and Applications March 2004Part I. OverviewStructural
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