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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
C0875
- 库存:
期货
- 供应商:
上海善然生物科技有限公司
ApplicationAs part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.Other NotesCultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.Cell Line OriginUK Caucasian FemaleCulture MediumRPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.Cell Line DescriptionA B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
extracted from liquid blood is not adversely affected by storage at 4ºC for up to 24 h [3]. Small but significant changes have been observed in metabonomic studies in samples of blood maintained at 48°C for 36 h [4]. DNA has been extracted from leucocytes
Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow
Regulation of blood flow in tissues such as skeletal muscle, liver, and adipose tissue is needed to meet the changing local metabolic and physiological demands under varying conditions. In healthy individuals, adipose tissue blood flow (ATBF
Isolation of Human Neutrophils From Venous Blood
Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes
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