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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
- 保质期:
详见说明
- 英文名:
Recombinant Matrix Metalloproteinase 9 (MMP9)
- 库存:
充足
- 供应商:
cloud-clone corp(优尔)
- 规格:
10ug
基质金属蛋白酶9(MMP9)重组蛋白
Purity≥ 95%;Fragment:Gly213~Ala399; Predicted Molecular Mass(KD):24.3kDa
优尔生的一级代理:杭州昊鑫生物
优尔生位于武汉出口加工区,已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证,以提供生命科学科研用检测试剂为主,75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存,能够做到约11,000种蛋白、19,000种抗体以及71565种检测试剂盒立等可取。蛋白项目部拥有四大技术平台,包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核(E.Coli)表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞)表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制,主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制,包括原料检测、半成品检测及成品检测。
- 编号RPA553Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌, 细胞外基质
- 预测分子量21.7kDa
- 实际分子量20kDa(差异分析请参阅说明书)
- 片段与标签Gly213~Ala399 with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
- 性状冻干粉
- 纯度> 95%
- 等电点5.6
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg 200µg 1mg 5mg
产品包装图(模拟):


用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
参考文献
| 杂志 | 参考文献 |
| Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice [PubMed: 21119180] |
| Journal of Surgical Research | Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia [ScienceDirect: S0022480409001942] |
| Journal of Proteome Research | The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9 [ACS: pr2006395] |
| Zahedan Journal of Research in Medical Sciences | The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source] |
| Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435] |
| PLoS One | Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Rheumatology International | Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model [Pubmed: 23408150] |
| Clinical and Investigative Medicine | The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells [Pubmed: 23544611] |
| Acta Histochemica | RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells [Pubmed: 24681113] |
| International journal of colorectal disease | Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair [Pubmed: 23619615] |
| Journal of Periodontology | Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial [Joponline: 130464] |
| Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Int J Clin Exp Med. | Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model [Pubmed:Pmc4132129] |
| The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
| Journal of Perinatology | Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion [Pubmed:25078863] |
| World J Emerg Med | Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9 [Pubmed:25215164] |
| PLOS ONE | Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model [Pubmed:25314292] |
| Stem Cells International | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513] |
| Physiology and Pharmacology | A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source] |
| Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 [Content: Asp] |
| PLOS | Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat [PubMed: 25945502] |
| Biomedical Materials | In vivo [PubMed: 25634132] |
| Histol Histopathol | Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells [PubMed: 26254457] |
| Int J Clin Exp Pathol | Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways [PubMed: 26191138] |
| Hattori and Ishihara Stem Cell Research & Therapy | Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells [PubMed: 25884474] |
| Neuropeptides | Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat [PubMed: 25616531] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140] |
| Canadian Journal of Physiology and Pharmacology | Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats [Doi: Abs] |
| PLASTIC AND AESTHETIC RESEARCH | A comparative study to evaluate the effect of limited access dressing on diabetic ulcers [Article: 2347-9264] |
| Kyselov | Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … [Article: View] |
| Оригинальные исследования / Original researches | матриксу та ранн [Article: Viewfile] |
| Int J Mol Sci | Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ [PubMed: 26703570] |
| Deletion Polymorphism and Chronic Obstructive Pulmonary Disease | ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells [Pubmed:27075970] |
| Basic Research | CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma [Journal:27699] |
| Journal of Cellular and Molecular Medicine | Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptor [Pubmed:27196940] |
| Journal of Stroke and Cerebrovascular Diseases | Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats [Pubmed:26851971] |
| Journal of the Science of Food and Agriculture | Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells [Pubmed:26991449] |
| Cell Physiol Biochem. | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 [Pubmed:27160777] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
| Onco Targets Ther. | sequential measurements of serum matrix metalloproteinase 9 to monitor chemotherapy responses in patients with advanced non-small-cell lung cancer [pmc:PMC4898419] |
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