基质金属蛋白酶7(MMP7)重组蛋白

基质金属蛋白酶7(MMP7)重组蛋白

Purity≥ 92%；Fragment：Met1~Lys267； Predicted Molecular Mass(KD)：33.3kDa

优尔生的一级代理：杭州昊鑫生物

优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,658种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。

• 编号RPA102Hu01
• 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
• 来源原核表达
• 宿主E.coli
• 内毒素水平<1.0EU/µg(LAL法测定)
• 亚细胞定位分泌, 细胞外基质
• 预测分子量33.3kDa
• 实际分子量33kDa(差异分析请参阅说明书)
• 片段与标签Met1~Lys267 with N-terminal His Tag
• 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
• 性状冻干粉
• 纯度> 90%
• 等电点8.3
• 应用Positive Control; Immunogen; SDS-PAGE; WB.
  如果需要有生物活性的蛋白，请参见活性蛋白。
• 下载英文说明书 中文说明书
• 规格10µg50µg 200µg 1mg 5mg

产品包装图（模拟）

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

参考文献

杂志	参考文献
British Journal of Pharmacology	A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin model [PubMed: 20649573]
Neuroimmunomodulation	Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source]
PLoS ONE	A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Thromb Haemost.	Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Histol Histopathol	Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells [PubMed: 26254457]
VirusDisease	Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446]
Am J Physiol Lung Cell Mol Physiol	Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522]
Cellular Physiology and Biochemistry	The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019]
s-space	Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708]
Science Translational Medicine	Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. [pubmed:29167395]
Hepatology	Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary Atresia [Pubmed: 30153340]
Journal of Pediatrics	Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary Atresia [Pubmed: 30853207]
Pediatrics	Serum MMP-7 in the Diagnosis of Biliary Atresia [Pubmed: 31604829]
Biomed Pharmacother	Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats [Pubmed: 31710895]
LABORATORY INVESTIGATION	The synthetic toxin biliatresone causes biliary atresia in mice [Pubmed: 32681026]
diagnostics	Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­ [33924683]
Cancer Cell Int	A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]