血管紧张素转化酶2(ACE2)重组蛋白

血管紧张素转化酶2(ACE2)重组蛋白

Recombinant Angiotensin I Converting Enzyme 2 (ACE2)

ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15

优尔生的一级代理：杭州昊鑫生物

优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及74109种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。
 

• 编号RPB886Mu01
• 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
• 来源原核表达
• 宿主E.coli
• 内毒素水平<1.0EU/µg(LAL法测定)
• 亚细胞定位细胞膜, 分泌, 细胞质
• 预测分子量46.6kDa
• 实际分子量44kDa(差异分析请参阅说明书)
• 片段与标签Ile93~Phe464 with N-terminal His Tag
• 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
• 性状冻干粉
• 纯度> 90%
• 等电点6.0
• 应用Positive Control; Immunogen; SDS-PAGE; WB.
  如果需要有生物活性的蛋白，请参见活性蛋白。
• 下载英文说明书   中文说明书
• 规格10µg50µg 200µg 1mg 5mg
• 价格¥ 1099

产品包装图模拟

• 产品包装(模拟)
• 产品包装(模拟)

1. 1
2. 2

• SDS-PAGE图

1. 1

• 通过ISO 9001、ISO 13485质量体系认证

1. 1

序列

 

 

 

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

参考文献

杂志	参考文献
Nature Medicine	Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu]
Journal of Cancer Therapy	Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
Reprod Toxicol.?	ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Intensive Care Medicine Experimental	Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Journal of Chromatography A	Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Journal of Clinical Pharmacy and Therapeutics	Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159]
Innate Immunity	Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931]
Journal of Surgical Research	Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
canadian journal of physiology and pharmacology	Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? [Pubmed: 30840489]
Intensive Care Medicine	Angiotensin-converting enzymes in acute respiratory distress syndrome []
BioMed Research International	AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway []
Heart and vessels	Relationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease [Pubmed: 31359146]
Medicine	Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine	Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Clinical Research in Cardiology	Determinants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease [33280062]
Clinical & Translational Immunology	Pattern of circulating SARS©\CoV©\2©\specific antibody©\secreting and memory B©\cell generation in patients with acute COVID©\19 [33552508]
researchsquare	Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis [33855640]
Viruses	Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera []
Sci Rep	Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury [34907257]
European Journal of Clinical Investigation	Angiotensin‐converting enzyme 2 and Transmembrane protease serine 2 in female and male patients with end‐stage kidney disease [Pubmed:35366343]
Metabolites	Ramipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats [Pubmed:35448480]