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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详见说明
- 库存:
详询
- 供应商:
上海古朵生物科技有限公司
- 规格:
10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume)
PackSize:10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume)
ProductName:FastStart TaqMan Probe Master
供应商:上海古朵生物科技公司
Roche
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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Real-Time PCR: the TaqMan Method
utilizes a system that is fairly easy to grasp conceptually. First, we must take a look at the TaqMan® probe. Figure 1. The Taqman probe. The red circle represents the quenching dye that disrupts the observable signal from the reporter dye
forward primer Taqman probe 5’ → 3’ 5’ FAM→3’染料 NNNNNNN NNNNNNN 发表序列:5’-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN-3’ 互补发表序列 3’-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN-5’ NNNNNNN 3’ ←5’ reverse primer 或: reverse
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