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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-26 (IL26)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Interleukin-26 (IL26)品名简称:
Recombinant Human IL26 protein货号:
CSB-YP873613HU规格:
20μg价格:
1500LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
IL26Uniprot No.:
Q9NPH9别名:
Protein AK155种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
19.6 kDa表达区域:
22-171aa氨基酸序列:
KHKQSSFTKSCYPRGTLSQAVDALYIKAAWLKATIPEDRIKNIRLLKKKTKKQFMKNCQFQEQLLSFFMEDVFGQLQLQGCKKIRFVEDFHSLRQKLSHCISCASSAREMKSITRMKRIFYRIGNKGIYKAISELDILLSWIKKLLESSQ蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin功能2:
May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin.文献:
"Induction of a novel cellular homolog of interleukin-10, AK155, by transformation of T lymphocytes with herpesvirus saimiri."Knappe A., Hor S., Wittmann S., Fickenscher H.J. Virol. 74:3881-3887(2000)研究领域:
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文献和实验采用亲和加分子筛两种层析方法就能得到大量高纯度的蛋白样品往往是别人家的实验。到自己手上总会有各种惊喜… 今天的主角更加过分,在真正纯化之前就需要我们施展十八般武艺把它从细胞上「拽」下来。 没错,它就是膜蛋白… 人类基因组中,有大约 30% 的基因编码膜蛋白。当今已批准上市的药物中,有超过 50% 以膜蛋白为靶点,然而目前蛋白质结构数据库 (Protein Data Bank, PDB) 中膜蛋白比例不足 1%。 欲了解其功能,必解析其结构。是很多结构生物学家心头的念念不忘,随着冷冻
,我们用已知的人工合成 MAP 作为阳性对照,分析研究拟南芥的不同 MAP 激酶 [23] 。 ( 4 ) 重组蛋白质除了含有各自的蛋白质的编码序列外,常常还包含由克隆载体所编码的 3' 或 5' 标记物。这些标记物的磷酸化可能会导致假阳性结果,因此在芯片实验之前要将其排除掉。用一些已知的不被认为是相应激酶底物,并且具有相同表达模式(相同标记物)的重组蛋白质,在溶液中进行活性激酶的检测实验可能是一种解决问题的办法。在这个检测实验中,这些所选择的蛋白质应该显示阴性
采用亲和加分子筛两种层析方法就能得到大量高纯度的蛋白样品往往是别人家的实验。到自己手上总会有各种惊喜… 今天的主角更加过分,在真正纯化之前就需要我们施展十八般武艺把它从细胞上「拽」下来。 没错,它就是膜蛋白… 人类基因组中,有大约 30% 的基因编码膜蛋白。当今已批准上市的药物中,有超过 50% 以膜蛋白为靶点,然而目前蛋白质结构数据库 (Protein Data Bank, PDB) 中膜蛋白比例不足 1%。 欲了解其功能,必解析其结构。是很多结构生物学家心头
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