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- 保存条件:
2-8 °C
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- 抗体英文名:
Llama anti-Mouse IgG (H&L), Affinity Pure
- 抗体名:
Llama anti-Mouse IgG (H&L), Affinity Pure
- 规格:
2.0 mg
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Immunostaining 24. Wash stored slides twice in PBS (10 min each wash). 25. Wash slides once in PBS containing 1% Triton X-100 for 10 min. 26. Incubate slides in a slide jar containing blocking solution for 1 h
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