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- 美国Immunoreagents Inc.
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- 2025年07月16日
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2-8 °C
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- 抗体英文名：
Chicken anti-Rat IgG (H&L) - Affinity Pure, FITC Conjugate, Cross-Adsorbed
- 抗体名：
Chicken anti-Rat IgG (H&L) - Affinity Pure, FITC Conjugate, Cross-Adsorbed
- 规格：
0.5 mg
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
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