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- 文献和实验
- 技术资料
- 保存条件:
低温/避光
- 库存:
大量
- 供应商:
上海晶抗生物工程有限公司
- CAS号:
17199-29-0
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文献和实验Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase
substituents in the reductive amination reaction. Optically pure (S)-phe and other (S)-amino acids are quantitatively synthesized from their oxo-analogs using PheDH, with a regeneration of NADH by formate dehydrogenase from Candida boidinii No. 2201. Acetone
have led to the conclusion that CYP2C19 is a major P450 responsible for (S)-mephenytoin 4’-hydroxylation activity in human liver microsomes (1 , 2 ). This is supported by the finding that cDNA-expressed CYP2C19 has a 50-to lOO-fold greater turnover number
A reverse-phase, high-performance liquid chromatography method is described for the quantification of 4′-hydroxymephenytoin formed enzymatically from 14 C-labeled (S )-mephenytoin following incubation with cDNA-expressed CYP2C19 or human
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