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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1527 |
---|---|
产品名称 | Human CD48 ELISA Kit |
产品描述 | For quantitative detection of human CD48 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q27-S220 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD48 |
基因全名 | CD48 molecule |
Uniprot ID | P09326 |
指标别名 | Antigen CD48 | BCM 1 surface antigen | Bcm-1 | BCM1 surface antigen | BCM1, BLAST1 | BLAST | CD48 antigen | CD48 molecule | CD48 protein | Leucocyte antigen MEM 102 | Leukocyte antigen MEM-102 | MEM102 | SLAMF2 | TCT.1 | P09326 |
背景资料 | CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1527-CAP | 96-well plate precoated with anti- human CD48 antibody | 1 |
EK1527-ST | Lyophilized recombinant human CD48 standard | 10ng/tube×2 |
EK1527-DA | Biotinylated anti- human CD48 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 113 | 615 | 2155 | 117 | 667 | 2219 |
Standard deviation | 5.53 | 46.12 | 129.3 | 6.66 | 58.69 | 157.54 |
CV(%) | 4.9% | 7.5% | 6% | 5.7% | 8.8% | 7.1% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.007 | 0.054 | 0.122 | 0.216 | 0.472 | 0.877 | 1.610 | 2.033 |
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