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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1429 |
---|---|
产品名称 | Human CD28 ELISA Kit |
产品描述 | For quantitative detection of human CD28 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, N19-P152 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD28 |
基因全名 | CD28 molecule |
Uniprot ID | P10747 |
指标别名 | CD 28 | CD28 | CD28 antigen | CD28 molecule | TP44 | P10747 |
背景资料 | CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1429-CAP | 96-well plate precoated with anti- human CD28 antibody | 1 |
EK1429-ST | Lyophilized recombinant human CD28 standard | 10ng/tube×2 |
EK1429-DA | Biotinylated anti- human CD28 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 187 | 557 | 1373 | 175 | 596 | 1354 |
Standard deviation | 13.27 | 25.06 | 82.38 | 15.05 | 33.37 | 82.59 |
CV(%) | 7.1% | 4.5% | 6% | 8.6% | 5.6% | 6.1% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.023 | 0.114 | 0.235 | 0.399 | 0.776 | 1.301 | 1.790 | 2.021 |
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