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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
96T
货号 | EK2032 |
---|---|
产品名称 | Human CHIT1 ELISA Kit |
产品描述 | For quantitative detection of human CHIT1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A22-N466 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CHIT1 |
基因全名 | chitinase 1 |
Uniprot ID | Q13231 |
指标别名 | Chitotriosidase-1; Chitinase-1; CHIT1 |
背景资料 | Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2032-CAP | 96-well plate precoated with anti- human CHIT1 antibody | 1 |
EK2032-ST | Lyophilized recombinant human CHIT1 standard | 20ng/tube×2 |
EK2032-DA | Biotinylated anti- human CHIT1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 590 | 1670 | 7933 | 594 | 1958 | 8471 |
Standard deviation | 30.68 | 71.81 | 325.25 | 39.20 | 113.56 | 559.09 |
CV(%) | 5.2% | 4.3% | 4.1% | 6.6% | 5.8% | 6.6% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.013 | 0.092 | 0.180 | 0.340 | 0.642 | 1.107 | 1.836 | 2.559 |
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