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PI3K p85 alpha Antibody抗体,orb2

16173,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb216173
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PI3K p85 alpha Antibody抗体

    • 抗体英文名

      PI3K p85 alpha Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb216173

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The PI3K p85 alpha Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500-1:1000, IHC-P: 1:100-1-200

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:25513, 5295, 18708

    UniProt ID:P27986, P26450, Q63787

    Note:For research use only.

    产品细节图片1
    Western blot analysis of PI3K p85 alpha expression in HEK293T (A), Hela (B), U2OS (C), mouse kidney (D) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 85 kD)

    产品细节图片2
    Immunohistochemical analysis of PI3K p85 alpha staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of PI3K p85 alpha staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • 【求助】p85和p-ERK western检测问题

      signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好,或者抗体不好,不知道还有没有可能是没有加磷酸化酶抑制剂,以至于磷酸化的蛋白被去磷酸化了? 谢谢啦~ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功? 用PLB裂解细胞得到的蛋白适合做REPORTER,做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。

    • Nat Commun:结直肠癌致癌突变 RNF43_pG659fs 对 PI3K/mTOR 抑制剂敏感

      标记进行定量蛋白质组学鉴定,结果发现 RNF43_p.G659fs 与 PI3K 调控亚基 p85 结合后将其泛素化降解,从而上调 AKT 的活性,提示 PI3K/mTOR 信号通路可做为 RNF43_p.G659fs 突变的治疗新靶点。 RNF43_p.G659fs 突变在微卫星不稳定 MSI 的患者中集中发生,但目前仍不清楚该突变和肿瘤免疫的关联。作者发现 RNF43_p.G659fs 与干扰素信号通路负相关,而且 PI3K/mTOR 抑制剂能逆转 p.G659fs 对干扰素信号通路的抑制

    • PI3K―Akt信号通路

      PI3K ― Akt 信号通路PI3K是一个复杂的大家族,根据其结构可分为3类:I型、Ⅱ型、Ⅲ型。 I型PI3K又分为IA和IB两个亚型,他们分别从酪氨酸激酶连接受体和G蛋白连接受体传递信号,其作用是催化磷脂酰肌醇(P1)在D3位的磷酸化,把底物PIP2转化为PIP3。IA型P13K是由催化亚单位Pll0和调节亚单位P85所组成的二聚体蛋白,具有类脂激酶和蛋白激酶的双重活性。在正常细胞中,P13K的活性是受许多机制严格控制的。一般认为,在静息细胞中,无活性的P85―P1lo复合物普遍

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