- 询价
- ICP0388
- 2025年07月15日
- ,
- rabbit
- ,
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- 文献和实验
- 技术资料
- 库存：
大量
- 是否单克隆：
2
- 抗原来源：
rabbit
- 应用范围：
,
- 适应物种：
,
- 宿主：
rabbit
- 规格：
0.5ml
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关于 Acetyl Lysine antibody, Agarose 的详细介绍
Acetyl Lysine antibody, Agarose
Product Description：The acetylated Lysine antibody is immobilized to the beaded agarose via amide linkage. The product could be utilized as affinity matrix for rapid isolation and purification of the species of protein or peptides with acetyl Lysine residues.
Species：Rabbit
Formulation： 0.5 mL beaded agarose suspended in 1 mL of glycerol.
Antibody immobilized： 2 mg/mL, antibody is covalently linked through amide bond with NHS activated-SMCC then linked to thiolated Agarose beads via thiol ether bond.
Storage & Stability： Store product at –20 ℃. One year from date of shipping.
Specificity: Selectively capture the peptides and proteins with acetylated lysine residues (N-epsilon). Not cross-react with methylated proteins and mono- and dimethylated proteins.
Binding Capacity: Approximately 0.2 mg of acetylated histone/mL.
Application(s): Rapid isolation and purification of peptides or proteins with acetylated lysine residues from the mixtures of cell lysates or protease-digested mixtures.
Scientific Description: Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation, and identification of the acetylated protein/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid method to enrich and purify the acetylated species for further MS/MS identification.
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文献和实验janetlee1976 我记得从前看到过检测细胞总体的蛋白质乙酰化水平的方法，大概是免疫荧光或者共聚焦显微之类的方法，现在找不到了，不知道哪位知道，请赐教（不是检测某种组蛋白或某种蛋白质的乙酰化）。可否提供参考文献？谢谢！ woxingwosu 免疫荧光后再共聚焦应该是可行的，或者是western也行。 目前除了蛋白特异性的乙酰化抗体以外，还有一些针对乙酰化赖氨酸（Lysine）的抗体Acetylated
巴豆酰化（Lysine Crotonylation, Kcr）存在于多种生物体的核心组蛋白和部分非组蛋白中，与其他类型的翻译后修饰(PTM) 类似，蛋白巴豆酰化（Lysine Crotonylation, Kcr）是一种可逆修饰，有促进基因表达、调控等重要功能。在蛋白质巴豆酰化中，最为常见的是赖氨酸巴豆酰化，由组蛋白乙酰转移酶 (HAT)、P300/CBP、PCAF 和 MOF 负责大多数巴豆酰化反应，而组蛋白去乙酰化酶HDAC1/2/3和SIRT1/2/3 逆转这些反应
Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
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