T细胞和B细胞活化miRNA 芯片T-Cell & B-Ce

“英拜为您实验加速”
技术服务网址：http://www.yingbio.com/
服务热线：400-696-6643、 18019265738
邮箱：daihp@yingbio.com 、 huizhang1228@foxmail.com

T-Cell & B-Cell Activation miScript miRNA PCR Array

T细胞和B细胞活化miRNA 芯片

Product	Species	Technology	Cat. No.
T-Cell & B-Cell Activation miScript miRNA PCR Array	Human	miRNA Expression	MIHS-111Z
T-Cell & B-Cell Activation miScript miRNA PCR Array	Mouse	miRNA Expression	MIMM-111Z
T-Cell & B-Cell Activation miScript miRNA PCR Array	Rat	miRNA Expression	MIRN-111Z

The Human T-Cell & B-Cell Activation miScript miRNA PCR Array profiles the expression of 84 miRNAs involved in the activation and differentiation of T cells and B cells. This array provides scientific researchers with a convenient way to quickly analyze the miRNAs most relevant to specific stages of T cell and B cell development. Hematopoietic stem cells differentiate into lymphoid and myeloid progenitor cells. To develop into T cells, lymphoid progenitor cells migrate to the thymus, where T cell lineage commitment starts with the double negative (CD4-/CD8-) stage. The cells proceed through the double positive (CD4+/CD8+) stage, and end with the single positive stage, generating either naïve CD4+ or naïve CD8+ T cells. During the adaptive immune response, naïve CD8+ T cells interact with antigen and activate, differentiating into effector T cells and potentially memory T cells after antigen clearance, whilenaïve CD4+ T cells activated by antigen differentiate into helper T cells. B cells mature in the spleen and remain naïve until they encounter antigen. Activated B cells differentiate into plasma B cells or memory B cells, or undergo an intermediate differentiation step and switch antibody classes to become germinal center B cells. T cell and B cell activation involves many sequential steps and the careful timing of cellular signaling pathways. Specific miRNAs play roles in fine-tuning the differentiation and activation of these essential immune cells, and dysregulation of B cell or T cell functions can cause immunodeficiencies or cancer.A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs involved in T cell and B cell activation can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
T细胞和B细胞活化miRNA 芯片用于研究参与T细胞和B细胞的活化和分化的84 miRNA的表达。这个芯片为科研人员提供了一个方便快速的方法来分析特定阶段T细胞和B细胞发育最相关的miRNA。造血干细胞分化成淋巴和髓系祖细胞。淋巴祖细胞迁移到胸腺, 保证T细胞谱系从双阴性(CD4-/CD8-)阶段开始，发展成T细胞。通过双阳性细胞(CD4 + / CD8 +)阶段,以单一阳性阶段结束,生成未分化T细胞CD4 +或CD8 +。在适应性免疫反应过程, CD8 + T细胞与抗原相互作用和激活,分化成效应T细胞和潜在的记忆T细胞,而CD4 + T细胞被抗原激活分化成辅助T细胞。B细胞在脾中成熟并保持未分化状态,直到它们遇到抗原。激活B细胞分化成血浆B细胞或记忆B细胞,或经过一个中间分化的步骤和转换抗体类型成为生发中心B细胞。T细胞和B细胞的激活涉及一序列的步骤和仔细的校时信号通路。特定的miRNA在微调重要免疫细胞的分化和激活中扮演重要角色,B细胞或T细胞功能的失调可以导致免疫缺陷或癌症。张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地检测参与T细胞和B细胞的激活的miRNA的表达。
miRNA PCR芯片用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。

T-Cell Differentiation*:
Double Negative (CD4-/CD8-):let-7d-5p, let-7e-5p, miR-126-3p, miR-128, miR-146b-5p, miR-15a-5p, miR-17-5p, miR-17-3p, miR-181c-5p, miR-191-5p, miR-19a-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-221-3p, miR-222-3p, miR-223-3p, miR-28-5p, miR-29c-3p, miR-30e-5p, miR-342-3p, miR-423-5p, miR-93-5p, miR-98-5p.
Double Positive (CD4+/CD8+):let-7b-5p, miR-181a-5p, miR-181b-5p, miR-181d, miR-19b-3p.
CD4+ Naïve:miR-132-3p, miR-146a-5p, miR-182-5p, miR-184, miR-25-3p, miR-326, miR-92a-3p.
CD8+ Naïve:let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-130b-3p, miR-139-5p, miR-142-3p, miR-150-5p, miR-155-5p, miR-15a-3p, miR-16-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-30b-5p, miR-30c-5p, miR-30d-5p.
CD8+ Effector:miR-147a, miR-148a-3p, miR-18a-5p, miR-27a-3p, miR-27b-3p.
CD8+ Memory:let-7i-5p, miR-106b-5p, miR-142-5p, miR-15b-5p, miR-17-5p, miR-21-5p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-29a-3p, miR-31-5p.
B-Cell Differentiation*:
Naïve:let-7a-5p, let-7b-5p, let-7d-5p, let-7g-5p, let-7i-5p, miR-101-3p, miR-132-3p, miR-142-3p, miR-142-5p, miR-150-5p, miR-181c-5p, miR-195-5p, miR-204-5p, miR-214-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-29b-3p, miR-30e-5p, miR-331-3p, miR-92a-3p.
Germinal Center:miR-106b-5p, miR-130b-3p, miR-132-3p, miR-148a-3p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-17-3p, miR-181a-5p, miR-181b-5p, miR-191-5p, miR-19a-3p, miR-19b-3p, miR-210, miR-23b-3p, miR-25-3p, miR-28-5p, miR-30d-5p, miR-93-5p, miR-98-5p.
Memory:miR-100-5p, miR-125b-5p, miR-145-5p, miR-146a-5p, miR-155-5p, miR-21-5p, miR-23a-3p, miR-24-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29c-3p, miR-30b-5p, miR-30c-5p, miR-34a-5p.
Differentially Expressed in Tregs:miR-100-5p, miR-125b-5p, miR-146a-5p, miR-181c-5p, miR-20b-5p, miR-21-5p, miR-31-5p, miR-335-5p, miR-365a-3p, miR-99a-5p.
T-Cell Activation:let-7e-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-139-5p, miR-142-5p, miR-146a-5p, miR-146b-5p, miR-155-5p, miR-15a-5p, miR-15a-3p, miR-181a-5p, miR-181c-5p, miR-195-5p, miR-20b-5p, miR-214-3p, miR-223-3p, miR-23a-3p, miR-23b-3p, miR-25-3p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-342-3p, miR-346, miR-574-3p, miR-98-5p.

What miScript miRNA PCR Arrays offer?
Guaranteed Performance* - ready-to-use for gene expression analysis
Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template