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T-Cell Activation: Regulators of T-Cell Activation: CD2, CD276, CD47, DPP4, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, CD8A, CD8B, FOXP3, ICOSLG, IRF4, LAG3, LCK, MAP3K7 (TAK1), MICB, NCK1, TNFSF14, VAV1.
T-Cell Prolifeion: CD28, CD3E, ICOSLG, IL1B, IL10, IL12B, IL18, NCK1, RIPK2, TNFSF14.
T-Cell Differentiation: ADA, APC, BCL2, BLM, CD1D, CD2, CD27 (TNFRSF7), CD4, CD80, CD86, EGR1, IL12B, IL15, IL2, IRF4, NOS2 (iNOS), PTPRC, SOCS1.
T-Cell Polarization: CCL3, CCR1, CCR2, CCR3, CCR4, CCR5, CD274, CD28, CD4, CD40LG (TNFSF5), CSF2 (GM-CSF), CXCR3, CXCR4, IFNG, IL12A, IL12RB1, IL12RB2, IL18R1, IL2, IL4, IL4R, IL5, TGFB1.
Regulators of Th1 and Th2 Development: CD2, CD40 (TNFRSF5), CD5, CD7, CSF2 (GM-CSF), IFNG, IL10, IL12A, IL13, IL3, IL4, IL5, TLR2, TLR4, TLR9.
Th1 & Th2 Differentiation: CD28, CD40 (TNFRSF5), CD40LG (TNFSF5), CD86, IFNG, IL12A, IL12B, IL12RB1, IL12RB2, IL18, IL18R1, IL2, IL2RA, IL4, IL4R, IL6.
B-Cell Activation: Antigen Dependent B-cell Activation: CD28, CD4, CD40 (TNFRSF5), CD40LG (TNFSF5), CD80, FAS (TNFRSF6), FASLG (TNFSF6), IL10, IL2, IL4.
Other Genes involved in B-Cell Activation: ADA, CXCR5, ICOSLG, IL6, IL7, MS4A1, TGFB1.
B-Cell Prolifeion: BCL2, CD27 (TNFRSF7), CD40 (TNFRSF5), CD81, IL10, IL7, PTPRC.
B-Cell Differentiation: ADA, AICDA, BLNK, CD27 (TNFRSF7), IL10, IL11, IL4, RAG1.
Other Immune Cell Activation: Macrophage Activation: IL13, IL4, TLR1, TLR4, TLR6.
Neutrophil Activation: IL8.
Natural Killer Cell Activation: CD2, IL12A, IL12B, IL2.
Leukocyte Activation: CX3CL1.
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文献和实验2-4 days incubation Method Prepare PBMC and resuspend the cells at 1-2x106/ml of complete RPMI. Note: This density of PBMC gives a good response. If experimental conditions require, a titration of cell densities (2-3x106/ml to 105/ml
1. T、B细胞相互作用的过程T细胞为Th2亚群，而B细胞既是Th细胞辅助的对象，又是T细胞活化的APC。这一相互合作包括一系列过程：①抗原以其B表位(绿色)与BCR结合传递B细胞活化的第一信号，使其C1340与细胞因子受体的表达增加，并使B细胞加工提呈抗原和表达少量D7分子；②B细胞作为APC将带有T表位(红色)的抗原肽提呈给CD4T细胞，供T细胞识别；③初步活化的T细胞表达CD40L等，与B细胞表面CD40结合成为B细胞活化的第二信号；④活化B细胞表达B7分子与CD28结合为T细胞提供第二
T-Cell Activation Using mAb to CD3
in clonal expansion of T cells upregulation of activation markers on the cell surface differentiation into effector cells induction of cytotoxicity or cytokine secretion induction of apoptosis
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