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- Cell Signaling Technology
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- 2025年07月15日
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文献和实验Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis
hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common
Detection of Apoptosis Using Cyclic Luciferase in Living Mammals
is composed of an engineered firefly luciferase, of which the N- and C-terminal ends are linked with a substrate sequence of caspase-3 (Asp-Glu-Val-Asp). When activated caspase-3 digests the substrate sequence, the cyclized luciferase recovers its activity
Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes
and represents an early physiologic marker of apoptosis. Here we present an immunoblotting technique that allows the detection of caspase-3 processing in cultured human chondrocytes. Apoptosis leads to plasma membrane asymmetry and to externalization
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