Phospho-c-Jun (Ser63) (54B3) R

5464:

Western Blotting

Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (Biotinylated) detects endogenous levels of c-Jun only when phosphorylated at Ser63.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun protein.

Western Blotting

Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999.

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-c-Jun (Ser63) (54B3) Rabbit mAb #2361.

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 3999 Streptavidin-HRP
• 2361 Phospho-c-Jun (Ser63) (54B3) Rabbit mAb
• 9260 PhosphoPlus® c-Jun (Ser63) and c-Jun (Ser73) Antibody Kit
• 9261 Phospho-c-Jun (Ser63) II Antibody
• 9263 c-Jun Control Cell Extracts
• 9164 Phospho-c-Jun (Ser73) Antibody
• 9165 c-Jun (60A8) Rabbit mAb
• 2315 c-Jun (L70B11) Mouse mAb
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.